Product Name | Goat anti-Fhl1/ Slim1 (mouse) Antibody |
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Description | This antibody is expected to recognize Mouse NP_001070830.1 iso 2 , NP_001274729.1 iso 3 and Rat NP_001029098.1 iso 1, NP_001258128.1 iso 3. |
Synonyms | FHL1; SLIM1; KYO-T; RP11-535K18.1; FHL1A; four and a half LIM domains 1; Four-and-a-half LIM domains 1; skeletal muscle LIM-protein1; FHL1B; KYOT; MGC111107; XMPMA; SLIMMER; LIM protein SLIMMER; four-and-a-half Lin11; Isl-1 and Mec-3 domains 1; skeletal m |
Host | Goat |
Clonality | Polyclonal |
Conjugate | Unconjugated |
Immunogen | Peptide with sequence CNKRFVFHNEQVY, from the internal region of the protein sequence according to NP_001070830.1; NP_001274729.1. |
Molecular Weight | 31.9; 33.6 |
Target | Fhl1/ Slim1 (mouse) |
Amino Acid Sequence | CNKRFVFHNEQVY |
Reactivity | Mouse, Rat |
Applications | ELISA, WB |
Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:32000. |
Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Reconstitution | WB: Approx 32kDa band observed in Mouse and Rat Skeletal Muscle and in Rat Lung lysates (calculated MW of 31.9kDa according to Mouse NP_001274729.1 and Rat NP_001258128.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature |
Gene Id | Non-Human GeneIDs: 14199 (mouse); 25177 (rat); |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
References | Brown S, McGrath MJ, Ooms LM, Gurung R, Maimone MM, Mitchell CA. , Characterization of two isoforms of the skeletal muscle LIM protein 1, SLIM1. Localization of SLIM1 at focal adhesions and the isoform slimmer in the nucleus of myoblasts and cytoplasm of myotubes suggests distinct roles in the cytoskeleton and in nuclear, J Biol Chem. 1999 Sep 17;274(38):27083-91., PMID: 10480922 |
Supplier | Everest Biotech |
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