| Product Name | Goat anti-FOXA1 / HNF3A Antibody |
|---|---|
| Synonyms | FOXA1; HNF3A; forkhead box A1; TCF3A; MGC33105; hepatocyte nuclear factor 3, alpha |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | C-GVYSRPVLNTS |
| Molecular Weight | 49.1 |
| Target | FOXA1 / HNF3A |
| Amino Acid Sequence | GVYSRPVLNTS |
| Reactivity | Bovine, Human, Mouse, Porcine, Rat |
| Applications | ELISA, FC, IF, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:128000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Approx 50kDa band observed in nuclear lysates of cell line HepG2 and approx 50-55kDa in Mouse Liver and in nuclear lysates of cell line MCF7 calculated MW of 49.1kDa according to Human NP_004487.2 and 48.9kDa according to Mouse NP_032285.2).These bands we. Notes: Immunofluorescence: Strong expression of the protein seen in the nuclei of MCF7 and U2OS cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10u |
| Gene Id | Human GeneIDs: 3169. Non-Human GeneIDs: 15375 (mouse); 25098 (rat); |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Lin L, Miller CT, Contreras JI, Prescott MS, Dagenais SL, Wu R, Yee J, Orringer MB, Misek DE, Hanash SM, Glover TW, Beer DG., The hepatocyte nuclear factor 3 alpha gene, HNF3alpha (FOXA1), on chromosome band 14q13 is amplified and overexpressed in esophageal and lung adenocarcinomas., Cancer Res. 2002 Sep 15;62(18):5273-9., PMID: 12234996 |
| Supplier | Everest Biotech |
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