| Product Name | Goat anti-Frizzled 2 Antibody |
|---|---|
| Synonyms | FZD2 ; frizzled homolog 2 (Drosophila) ; frizzled 2 |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | RYATLEHPFHCPR |
| Molecular Weight | 63.6 |
| Target | Frizzled 2 |
| Amino Acid Sequence | RYATLEHPFHCPR |
| Reactivity | Human, Mouse, Rat |
| Applications | ELISA, IF, IHC, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:64000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Approx 60-65kDa band observed in Human Heart and Placenta lysates (calculated MW of 63.6 kDa according to NP_001457.1) An additional band of approx. 24kDa was also observed in some lysates which was successfully blocked by incubation with the immunizing . IHC: In paraffin embedded Human Thyroid Gland shows cytoplasm and membranous staining in epithelial cells of the follicles. Recommended concentration: 2-5µg/ml. Paraffin embedded Human Liver. Recommended concentration: 2.5µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U2OS cells. Recommended concentration: 10µg/ml. |
| Gene Id | Human GeneIDs: 2535. Non-Human GeneIDs: 57265 (mouse); 64512 (rat); |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Oishi I, Kawakami Y, Raya A, Callol-Massot C, Izpisua Belmonte JC., Regulation of primary cilia formation and left-right patterning in zebrafish by a noncanonical Wnt signaling mediator, duboraya., Nat Genet. 2006 Nov;38(11):1316-22. Epub 2006 Oct 1., PMID: 17013396 |
| Supplier | Everest Biotech |
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