| Product Name | Goat anti-GATA1 Antibody |
|---|---|
| Synonyms | GATA1; GATA binding protein 1 (globin transcription factor 1); HGNC:4170; ERYF1; GF1; NFE1; GATA binding protein 1; GATA-binding protein 1 (globin transcription factor 1); erythrold transcription factor 1; globin transcription factor 1; transcription fact |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | C-DAEAYRHSPVFQ |
| Molecular Weight | 42.8 |
| Target | GATA1 |
| Amino Acid Sequence | DAEAYRHSPVFQ |
| Reactivity | Bovine, Canine, Human, Mouse, Rat |
| Applications | ELISA, FC, IF, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:16000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Approx 50-55kDa band observed in nuclear lysates of cell line K562 calculated MW of 42.8kDa according to NP_002040.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room t. Notes: Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and NIH3T3 cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 1 |
| Gene Id | Human GeneIDs: 2623 |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Li Z, Godinho FJ, Klusmann JH, Garriga-Canut M, Yu C, Orkin SH. , Developmental stage-selective effect of somatically mutated leukemogenic transcription factor GATA1., Nat Genet. 2005 Jun;37(6):613-9. Epub 2005 May 15. , PMID: 15895080 |
| Supplier | Everest Biotech |
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