Goat anti-Gelsolin / GSN Antibody

Category: Antibodies
Catalog
EB07503
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Product Name Goat anti-Gelsolin / GSN Antibody
Synonyms GSN; ; gelsolin; gelsolin (amyloidosis, Finnish type) ; DKFZp313L0718
Host Goat
Clonality Polyclonal
Conjugate Unconjugated
Immunogen C-PRLKDKKMDAHP
Molecular Weight 85.7; 80.6; 81.9; 82.5; 81.5; 84.7; 83.3; 56.0
Target Gelsolin / GSN
Amino Acid Sequence PRLKDKKMDAHP
Reactivity Bovine, Canine, Human, Mouse, Rat
Applications ELISA, FC, WB
Working Dilutions Peptide ELISA: antibody detection limit dilution 1:128000.
Form Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Diluent Buffer Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Reconstitution WB: Approx 90kDa observed in Human Colorectal cancer lysates and in prelominary testing of Human Spleen and Parathyroid lysate (calculated MW of 85.7kDa according to NP_000168.1). Recommended concentration: 0.1-2µg/ml. Primary incubation 1 hour at room temp. Notes: Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
Gene Id Human GeneIDs: 2934. Non-Human GeneIDs: 227753 (mouse); 296654 (rat);
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
References Tanaka H, Shirkoohi R, Nakagawa K, Qiao H, Fujita H, Okada F, Hamada J, Kuzumaki S, Takimoto M, Kuzumaki N. , siRNA gelsolin knockdown induces epithelial-mesenchymal transition with a cadherin switch in human mammary epithelial cells., Int J Cancer. 2006 Apr 1;118(7):1680-91. , PMID: 16217750
Supplier Everest Biotech

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