| Product Name | Goat anti-GPR125 Antibody |
|---|---|
| Synonyms | GPR125 ; G protein-coupled receptor 125 ; PGR21; TEM5L; TEM5-like |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | CTNKSASSFKNS |
| Molecular Weight | 146 |
| Target | GPR125 |
| Amino Acid Sequence | CTNKSASSFKNS |
| Reactivity | Human, Mouse |
| Applications | ELISA, IHC, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:8000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Approx 140kDa band observed in Human Ovary lysates (calculated MW of 146kDa according to NP_660333.2). Recommended concentration: 0.5-1.5µg/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 33kDa. IHC: Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75µg/ml. A customer reported successful results on Human Testis. |
| Gene Id | Human GeneIDs: 166647. Non-Human GeneIDs: 70693 (mouse); |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Seandel M, James D, Shmelkov SV, Falciatori I, Kim J, Chavala S, Scherr DS, Zhang F, Torres R, Gale NW, Yancopoulos GD, Murphy A, Valenzuela DM, Hobbs RM, Pandolfi PP, Rafii S., Generation of functional multipotent adult stem cells from GPR125+ germline progenitors., Nature. 2007 Sep 20;449(7160):346-50., PMID: 17882221 |
| Supplier | Everest Biotech |
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