| Product Name | Goat anti-GPR94 / TRA1 Antibody |
|---|---|
| Synonyms | TRA1; GRP94; tumor rejection antigen (gp96) 1 ; HGNC:12028; ECGP; GP96; Tumor rejection antigen-1 (gp96); endothelial cell (HBMEC) glycoprotein; glucose regulated protein, 94 kDa; tumor rejection antigen (gp96) 1 |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | C-KEGVKFDESEKTKE |
| Molecular Weight | 92.5 |
| Target | GPR94 / TRA1 |
| Amino Acid Sequence | KEGVKFDESEKTKE |
| Reactivity | Bovine, Canine, Human, Mouse, Porcine, Rat, Zebrafish |
| Applications | ELISA, FC, IF, IHC, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:32000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Approx 100kDa band observed in lysates of cell lines HEK293, A549, HeLa, U2OS and NIH3T3 and in preliminary testing of MCF7 and HepG2 cell lysate (calculated MW of 92.5kDa according to Human NP_003290.1 and Mouse NP_035761.1). This molecular weight is rou. IHC: Paraffin embedded Human Uterus and Liver. Recommended concentration: 3-5µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the ER and cytoplasm of A431 and HeLa cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentr |
| Gene Id | Human GeneIDs: 7184. Non-Human GeneIDs: 22027 (mouse); 362862 (rat); |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Stojkovic M, Lako M, Stojkovic P, Stewart R, Przyborski S, Armstrong L, Evans J, Herbert M, Hyslop L, Ahmad S, Murdoch A, Strachan T. , Derivation of human embryonic stem cells from day-8 blastocysts recovered after three-step in vitro culture., Stem Cells. 2004;22(5):790-7. , PMID: 15342943 |
| Supplier | Everest Biotech |
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