| Product Name | Goat anti-HSPA5 / GRP78 (aa118-131) Antibody |
|---|---|
| Synonyms | HSPA5; heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); BIP; GRP78; HEL-S-89n; MIF2; 78 kDa glucose-regulated protein; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; epididymis secretory sperm binding protein Li 89n; immunoglobu |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | C-KVVEKKTKPYIQVD |
| Molecular Weight | 72.3 |
| Target | HSPA5 / GRP78 (aa118-131) |
| Amino Acid Sequence | KVVEKKTKPYIQVD |
| Reactivity | Human |
| Applications | ELISA, IF, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:128000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Approx 75kDa band observed in lysates of cell lines HepG2, HeLa and MCF7, and in Mouse Liver and Testis lysates, and approx. 80kDa band in Rat Brain lysates (calculated MW of 72.3kDa according to Human NP_005338.1 and Rat NP_037215.1, and 72.4kDa accordin. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 5µg/ml. |
| Gene Id | Human GeneIDs: 3309 |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Reid SP, Shurtleff AC, Costantino JA, Tritsch SR, Retterer C, Spurgers KB, Bavari S., HSPA5 is an essential host factor for Ebola virus infection., Antiviral research 2014 Sep 109 : 171-4., PMID: 25017472 |
| Supplier | Everest Biotech |
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