| Product Name | Goat anti-IRAK4 (N Terminus) Antibody |
|---|---|
| Synonyms | IRAK4; REN64; NY-REN-64; interleukin-1 receptor-associated kinase 4; IRAK-4 mutated form 1; interleukin-1 receptor associated kinase 4; interleukin-1 receptor associated kinase 4 mutant form 1; IPD1 |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | NKPITPSTYVRC |
| Molecular Weight | 51.5 |
| Target | IRAK4 (N Terminus) |
| Amino Acid Sequence | NKPITPSTYVRC |
| Reactivity | Human, Mouse |
| Applications | ELISA, FC, IF, IHC, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:32000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Approx 50kDa band observed in lysates of cell line HeLa and approx 55kDa in lysates of cell line Jurkat (calculated MW of 51.5kDa according to NP_057207.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.5-2µg/m. IHC: Paraffin embedded Human Heart and Human Liver. Recommended concentration: 3.75µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm and microtubules of HeLa cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concent |
| Gene Id | Human GeneIDs: 51135. Non-Human GeneIDs: 266632 (mouse); |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Lye E, Mirtsos C, Suzuki N, Suzuki S, Yeh WC. , The role of interleukin 1 receptor-associated kinase-4 (IRAK-4) kinase activity in IRAK-4-mediated signaling., J Biol Chem. 2004 Sep 24;279(39):40653-8. Epub 2004 Jul 29. , PMID: 15292196 |
| Supplier | Everest Biotech |
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