| Product Name | Goat anti-KIT / CD117 Antibody |
|---|---|
| Synonyms | KIT; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; C-Kit; CD117; PBT; SCFR; mast/stem cell growth factor receptor Kit; p145 c-kit; piebald trait protein; proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; soluble KIT varian |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | KRDSFICSKQEDH |
| Molecular Weight | 110; 109 |
| Target | KIT / CD117 |
| Amino Acid Sequence | KRDSFICSKQEDH |
| Reactivity | Bovine, Canine, Human, Porcine |
| Applications | ELISA, FC, IF, IHC, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:128000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Approx 110kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 110kDa according to NP_000213.1). An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the . IHC: Paraffin embedded Human Prostate. Recommended concentration: 10µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ membranes of HEK293 and HeLa cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended co |
| Gene Id | Human GeneIDs: 3815 |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Herpel E, Jensen K, Muley T, Warth A, Schnabel PA, Meister M, Herth FJ, Dienemann H, Thomas M, Gottschling S., The cancer stem cell antigens CD133, BCRP1/ABCG2 and CD117/c-KIT are not associated with prognosis in resected early-stage non-small cell lung cancer., Anticancer Res. 2011 Dec;31(12):4491-500., PMID: 22199321 |
| Supplier | Everest Biotech |
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