| Product Name | Goat anti-MAPK3/ERK1 Antibody |
|---|---|
| Synonyms | MAPK3; ERK1; mitogen-activated protein kinase 3; HGNC:6877; P44ERK1; P44MAPK; PRKM3; protein kinase, mitogen-activated 3 (MAP kinase 3 p44); HS44KDAP; HUMKER1A; MGC20180; OTTHUMP00000174538; extracellular signal-regulated kinase 1; extracellular signal-re |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | GGEPRRTEGVGP-C |
| Molecular Weight | 43.1; 40.1; 38.3 |
| Target | MAPK3/ERK1 |
| Amino Acid Sequence | GGEPRRTEGVGP |
| Reactivity | Human |
| Applications | ELISA, FC, IF, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:4000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Approx 40kDa band observed in Human Cerebellum lysates and in lysates of cell lines A431 and HepG2, and approx 42kDa obseved in Human Frontal Cortex lysates (calculated MW of 40.1kDa according to NP_001035145.1 and 43.1kDa according to NP_002737.2). Reco. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and A431 cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: |
| Gene Id | Human GeneIDs: 5595 |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Wang PY, Weng J, Anderson RG. , OSBP is a cholesterol-regulated scaffolding protein in control of ERK 1/2 activation., Science. 2005 Mar 4;307(5714):1472-6. , PMID: 15746430 |
| Supplier | Everest Biotech |
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