Goat anti-MAPKAPK2 (aa44-55) Antibody

Category: Antibodies
Catalog
EB11930
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Product Name Goat anti-MAPKAPK2 (aa44-55) Antibody
Synonyms MAPKAPK2; mitogen-activated protein kinase-activated protein kinase 2; MAPKAP-K2; MK-2; MK2; MAP kinase-activated protein kinase 2; MAPK-activated protein kinase 2; MAPKAP kinase 2
Host Goat
Clonality Polyclonal
Conjugate Unconjugated
Immunogen C-PQFHVKSGLQIK
Molecular Weight 42.2; 45.6
Target MAPKAPK2 (aa44-55)
Amino Acid Sequence PQFHVKSGLQIK
Reactivity Human, Mouse, Rat
Applications ELISA, IP, WB
Working Dilutions Peptide ELISA: antibody detection limit dilution 1:32000.
Form Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Diluent Buffer Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Reconstitution WB: In transfected HEK293 transiently expressing either Mouse MK2 fused to GFP or the similar Mouse MK5 fused to GFP, only the expected band was observed in the MK2 lane after EB11929 and EB11930 labeling. Data obtained from Dr. M. B. Menon, Inst. Biochemist. Notes: Immunoprecipitation: MK2/MK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced wiith MK2 or empty vector were lysed from confluent plates and used for IP with 1.5ug EB11929 or EB11930. Western blots of the IP were
Gene Id Human GeneIDs: 9261. Non-Human GeneIDs: 17164 (mouse); 289014 (rat);
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
References Yang Y, Xia F, Hermance N, Mabb A, Simonson S, Morrissey S, Gandhi P, Munson M, Miyamoto S, Kelliher MA., A cytosolic ATM/NEMO/RIP1 complex recruits TAK1 to mediate the NF-kappaB and p38 mitogen-activated protein kinase (MAPK)/MAPK-activated protein 2 responses to DNA damage., Mol Cell Biol. 2011 Jul;31(14):2774-86, PMID: 21606198
Supplier Everest Biotech

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