| Product Name | Goat anti-MK2 / MAPKAPK2 Antibody |
|---|---|
| Synonyms | MAPKAPK2; mitogen-activated protein kinase-activated protein kinase 2; MAPKAP-K2; MK-2; MK2; MAP kinase-activated protein kinase 2; MAPK-activated protein kinase 2; MAPKAP kinase 2 |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | C-SRVLKEDKERWED |
| Molecular Weight | 42.2; 45.6 |
| Target | MK2 / MAPKAPK2 |
| Amino Acid Sequence | SRVLKEDKERWED |
| Reactivity | Bovine, Canine, Human, Mouse, Rat |
| Applications | ELISA, IHC, IP, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:128000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Approx 60kDa band observed in Mouse Embryonic Fibroblasts (MEF) lysates, not present in the knockout MEFs and reappearing as a 45kDa band upon reintroduction of MK2 gene expression through viral transduction (calculated MW of 44.0kDa according to Mouse NP. IHC: Paraffin embedded Human Heart. Recommended concentration: 5µg/ml.. Notes: Immunoprecipitation: MK2/MK3 double knockout MEFs retrovirally transduced wiith MK2 or empty vector were lysed from confluent plates and used for IP with 1.5ug EB11929 or EB11930. Western blots of the IP were labelled with rabbit anti-MK2 |
| Gene Id | Human GeneIDs: 9261. Non-Human GeneIDs: 17164 (mouse); 289014 (rat); |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Yang Y, Xia F, Hermance N, Mabb A, Simonson S, Morrissey S, Gandhi P, Munson M, Miyamoto S, Kelliher MA., A cytosolic ATM/NEMO/RIP1 complex recruits TAK1 to mediate the NF-kappaB and p38 mitogen-activated protein kinase (MAPK)/MAPK-activated protein 2 responses to DNA damage., Mol Cell Biol. 2011 Jul;31(14):2774-86, PMID: 21606198 |
| Supplier | Everest Biotech |
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