| Product Name | Goat anti-MK5 / MAPKAPK5 (aa192-206) Antibody |
|---|---|
| Synonyms | MAPKAPK5; mitogen-activated protein kinase-activated protein kinase 5; MAPKAP-K5; MK-5; MK5; PRAK; MAP kinase-activated protein kinase 5; MAPK-activated protein kinase 5; MAPKAP kinase 5; MAPKAPK-5; p38-regulated/activated protein kinase |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | C-PQVLEAQRRHQKEKS |
| Molecular Weight | 54.0; 54.2 |
| Target | MK5 / MAPKAPK5 (aa192-206) |
| Amino Acid Sequence | PQVLEAQRRHQKEKS |
| Reactivity | Bovine, Canine, Human, Mouse, Porcine, Rat |
| Applications | ELISA, IHC, IP, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:64000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: In transfected HEK293 transiently expressing either Mouse MK5 fused to GFP or the similar Mouse MK2 fused to GFP , only the expected band was observed in the MK5 lane after EB11928 labeling. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannove. IHC: In paraffin embedded Human Kidney shows nuclear staining in the DCT. Recommended concentration, 4-8µg/ml.. Notes: Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced wiith MK5/ERK3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5ug EB11927 or EB11928. Western blots of |
| Gene Id | Human GeneIDs: 8550. Non-Human GeneIDs: 17165 (mouse); 498183 (rat); |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Yoshizuka N, Chen RM, Xu Z, Liao R, Hong L, Hu WY, Yu G, Han J, Chen L, Sun P., A novel function of p38-regulated/activated kinase in endothelial cell migration and tumor angiogenesis., Mol Cell Biol. 2012 Feb;32(3):606-18., PMID: 22124154 |
| Supplier | Everest Biotech |
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