| Product Name | Goat anti-NANOG Antibody |
|---|---|
| Synonyms | NANOG; Nanog homeobox; HGNC:20857 ; homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48 |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | C-QNQRMKSKRWQKNN |
| Molecular Weight | 34.6 |
| Target | NANOG |
| Amino Acid Sequence | QNQRMKSKRWQKNN |
| Reactivity | Canine, Human, Porcine |
| Applications | ELISA, IF, IHC, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:64000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Approx 38kDa band observed in Human Ovary lysates (calculated MW of 34.6kDa according to NP_079141.2). Recommended concentration: 0.03-0.1µg/ml. Primary incubation was 1 hour.. IHC: This antibody was successfully used in: Vassena et al, Hum Mol Genet. 2012 Aug 1;21(15):3366-73. PMID: 22547223, Sancho-Martinez et al, Nat Commun. 2016 Feb 22;7:10743. PMID: 26899176, and Montserrat et al, Cell Transplant. 2012;21(5):815-25. PMID: 219444. Notes: IF/ICC: Parts of a keratinocyte-derived colony of induced pluripotent stem cells are stained for Nanog (green). This antibody was successfully used in IF on Human: PMID: 30878013, 21681858, 22613719, 21877920. 21285354,19890879, 19483 |
| Gene Id | Human GeneIDs: 79923. Non-Human GeneIDs: 71950 (mouse); 414065 (rat); |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Hyslop L, Stojkovic M, Armstrong L, Walter T, Stojkovic P, Przyborski S, Herbert M, Murdoch A, Strachan T, Lako M., Downregulation of NANOG induces differentiation of human embryonic stem cells to extraembryonic lineages., Stem Cells. 2005 Sep;23(8):1035-43. Epub 2005 Jun 27., PMID: 15983365 |
| Supplier | Everest Biotech |
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