| Product Name | Goat anti-NCF1 / p47phox Antibody |
|---|---|
| Synonyms | NCF1; p47phox; FLJ79451; NCF1A; NOXO2; SH3PXD1A; neutrophil cytosolic factor 1; Neutrophil cytosolic factor-1 (47kD); neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal 1); NADPH oxidase organizer 2 |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | C-SESTKRKLASAV |
| Molecular Weight | 44.7 |
| Target | NCF1 / p47phox |
| Amino Acid Sequence | SESTKRKLASAV |
| Reactivity | Bovine, Human, Mouse, Porcine, Rat |
| Applications | ELISA, FC, IF, IHC, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:20000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Approx 48kDa band observed in lysates of cell lines Daudi, U251 and U937 and in Mouse Thymus, and approx, 50kDa in Pig Spleen lysates (calculated MW of 44.7kDa according to Human NP_000256.4, 46.0kDa according to Mouse NP_001272966.1 and 45.3kDa according. IHC: Paraffin embedded Human Colon, Neutrophils. Recommended concentration: 5µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.< |
| Gene Id | Human GeneIDs: 653361. Non-Human GeneIDs: 17969 (mouse); 114553 (rat); |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Davidson-Moncada JK, Lopez-Lluch G, Segal AW, Dekker LV, Involvement of protein kinase D in Fc gamma-receptor activation of the NADPH oxidase in neutrophils., Biochem J 2002 Apr 1;363(Pt 1):95-103, PMID: 11903052 |
| Supplier | Everest Biotech |
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