| Product Name | Goat anti-NEDD8 conjugating enzyme (N Terminus) Antibody |
|---|---|
| Synonyms | UBE2F; ubiquitin-conjugating enzyme E2F (putative); MGC18120; NCE2; NEDD8 carrier protein UBE2F; NEDD8 conjugating enzyme; NEDD8 protein ligase UBE2F; NEDD8-conjugating enzyme 2; NEDD8-conjugating enzyme UBE2F; OTTHUMP00000202749; OTTHUMP00000202755; OTTH |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | SKLKRDDGLKGSRT-C |
| Molecular Weight | 21.1 |
| Target | NEDD8 conjugating enzyme (N Terminus) |
| Amino Acid Sequence | SKLKRDDGLKGSRT |
| Reactivity | Bovine, Canine, Human, Mouse, Porcine, Rat |
| Applications | ELISA, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:32000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Approx 22kDa band observed in Rat Pancreas lysates (calculated MW of 21.1kDa according to Human NP_542409.1 and 18.6kDa according to Rat NP_001008382.1). Recommended concentration: 0.05-0.2µg/ml. |
| Gene Id | Human GeneIDs: 140739. Non-Human GeneIDs: 67921 (mouse); 363284 (rat); |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Huang DT, Ayrault O, Hunt HW, Taherbhoy AM, Duda DM, Scott DC, Borg LA, Neale G, Murray PJ, Roussel MF, Schulman BA., E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification., Mol Cell. 2009 Feb 27;33(4):483-95., PMID: 19250909 |
| Supplier | Everest Biotech |
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