| Product Name | Goat anti-NQO1 Antibody |
|---|---|
| Synonyms | NQO1; NAD(P)H dehydrogenase, quinone 1; DTD; QR1; DHQU; DIA4; NMOR1; NMORI; diaphorase-4; diaphorase (NADH/NADPH); diaphorase (NADH/NADPH) (cytochrome b-5 reductase); NAD(P)H:menadione oxidoreductase 1, dioxin-inducible 1; DT-diaphorase; NAD(P)H menadione |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | C-SIPTDNQIKARK |
| Molecular Weight | 30.9; 27.3; 26.4 |
| Target | NQO1 |
| Amino Acid Sequence | SIPTDNQIKARK |
| Reactivity | Canine, Human, Porcine, Rat |
| Applications | ELISA, IF, IHC, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:64000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Approx. 30kDa band observed in lysates of cell lines U251 and HepG2 and in Human Kidney, Lung and Duodenum lysates, and approx. 30-32kDa band in Rat Kidney, Lung and Duodenum lysates (calculated MW of 30.9kDa according to Human NP_000894.1 and Rat NP_0586. IHC: In paraffin embedded Human Kidney shows specific staining of the glomeruli. Recommended concentration: 2-4µg/ml.. Notes: Immunofluorescence: Expression of the protein seen in the cytoplasm and nucleus of HepG2 cells. Recommended concentration: 10µg/ml. |
| Gene Id | Human GeneIDs: 1728. Non-Human GeneIDs: 18104 (mouse); |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Asher G, Lotem J, Kama R, Sachs L, Shaul Y., NQO1 stabilizes p53 through a distinct pathway., Proc Natl Acad Sci U S A. 2002 Mar 5;99(5):3099-104., PMID: 11867746 |
| Supplier | Everest Biotech |
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