| Product Name | Goat anti-Nucleophosmin / NPM1 Antibody |
|---|---|
| Synonyms | NPM1; NPM; B23; nucleophosmin (nucleolar phosphoprotein B23, numatrin); numatrin; nucleolar phosphoprotein B23; nucleophosmin/nucleoplasmin family, member 1; MGC104254; nucleophosmin 1 |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | C-QEAIQDLWQWRKSL |
| Molecular Weight | 32.6; 29.5; 25.3; 18.9 |
| Target | Nucleophosmin / NPM1 |
| Amino Acid Sequence | QEAIQDLWQWRKSL |
| Reactivity | Bovine, Canine, Human, Mouse, Rat |
| Applications | ELISA, IF, IHC, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:8000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Approx 37kDa band observed in lysates of cell lines Daudi, Jurkat, K562 and NIH3T3 and in Mouse Spleen lysates (calculated MW of 32.6kDa according to Human NP_001341935.1 and Mouse NP_032748.1). This molecular weight is routinely observed by other sources. IHC: Paraffin embedded Human Testis. Recommended concentration: 5µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the nucleoli of HeLa cells. Recommended concentration: 10µg/ml. |
| Gene Id | Human GeneIDs: 4869 |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Grisendi S, Pandolfi PP. , NPM mutations in acute myelogenous leukemia., N Engl J Med. 2005 Jan 20;352(3):291-2., PMID: 15659732 |
| Supplier | Everest Biotech |
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