| Product Name | Goat anti-OGT Antibody |
|---|---|
| Synonyms | OGT; O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase); FLJ23071; HRNT1; MGC22921; O-GLCNAC ; O-GlcNAc transferase p110 subunit O-linked GlcNAc transferase; uridinediphospho-N-acetylgl |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | C-YEHPKDLKLSDGR |
| Molecular Weight | 117, 116 |
| Target | OGT |
| Amino Acid Sequence | YEHPKDLKLSDGR |
| Reactivity | Bovine, Canine, Human, Mouse, Rat |
| Applications | ELISA, FC, IF, IHC, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:64000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Approx 110kDa band observed in Rat Pancreas lysates calculated MW of 116kDa according to NP_858058.2). An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising pep. IHC: Paraffin embedded Human Brain (Cortex). Recommended concentration: 5µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the nucleus of HeLa, U2OS and Glioblastoma U251 cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recomme |
| Gene Id | Human GeneIDs: 8473. Non-Human GeneIDs: 108155 (mouse); 26295 (rat); |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Majumdar G, Wright J, Markowitz P, Martinez-Hernandez A, Raghow R, Solomon SS. , Insulin stimulates and diabetes inhibits O-linked N-acetylglucosamine transferase and O-glycosylation of Sp1., Diabetes. 2004 Dec;53(12):3184-92. , PMID: 15561949 |
| Supplier | Everest Biotech |
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