| Product Name | Goat anti-POU3F3 / BRN1 / OCT8 Antibody |
|---|---|
| Synonyms | POU3F3; POU class 3 homeobox 3; BRN1; OTF8; OTF-8; POU domain, class 3, transcription factor 3; brain-1; brain-specific homeobox/POU domain protein 1; brn-1; oct-8; octamer-binding protein 8; octamer-binding transcription factor 8 |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | C-HMLSHAHQWVTAL |
| Molecular Weight | 50.3 |
| Target | POU3F3 / BRN1 / OCT8 |
| Amino Acid Sequence | HMLSHAHQWVTAL |
| Reactivity | Human, Mouse, Rat |
| Applications | ELISA, FC, IF, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:4000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Approx 48kDa band observed in Mouse Spinal Cord lysates and approx. 50kDa in Mouse Brain lysates (calculated MW of 50.3kDa according to Human NP_006227.1 and 50.2kDa according to Mouse NP_032926.2). Recommended concentration: 1-3µg/ml. Primary incubation. Notes: Immunofluorescence: Strong expression of the protein seen in the nuclei of Neuro-2a cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of Neuro-2a cells. Recommended concentration: 10ug |
| Gene Id | Human GeneIDs: 5455. Non-Human GeneIDs: 18993 (mouse); 192109 (rat); |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Castro DS, Skowronska-Krawczyk D, Armant O, Donaldson IJ, Parras C, Hunt C, Critchley JA, Nguyen L, Gossler A, Göttgens B, Matter JM, Guillemot F, , Proneural bHLH and Brn proteins coregulate a neurogenic program through cooperative binding to a conserved DNA motif., Developmental cell 2006 Dec 11 (6): 831-44., PMID: 17141158 |
| Supplier | Everest Biotech |
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