| Product Name | Goat anti-PRKD1 (aa233-246) Antibody |
|---|---|
| Synonyms | PRKD1; PKC-MU; PKCM; PKD; PRKCM; |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | C-QKSPSESFIGREKR |
| Molecular Weight | 102 |
| Target | PRKD1 (aa233-246) |
| Amino Acid Sequence | QKSPSESFIGREKR |
| Reactivity | Human, Mouse, Porcine, Rat |
| Applications | ELISA, IHC, IP, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:128000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: In transfected HEK293 transiently expressing Human PKD1 (DYKDDDDK tagged), a band of approx. 100kDa is observed. The same band is observed using anti- DYKDDDDK antibody and no bands are observed in cells transiently expressing Human PKD2 or PKD3. Recommen. IHC: This antibody has been confirmed in human pancreatic adenocarcinoma (PDA) according to Döppler et al, Sci Rep. 2016 Sep 21;6:33758 PMID 27649783.. Notes: Immunoprecipitation: PKD1 was immunoprecipitated from Hek293T cells (as above). Immunoprecipitates were analyzed by Western Blot probed with the same antibody. |
| Gene Id | Human GeneIDs: 5587. Non-Human GeneIDs: 18760 (mouse); 85421 (rat); |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Borges S, Döppler H, Perez EA, Andorfer CA, Sun Z, Anastasiadis PZ, Thompson E, Geiger XJ, Storz P., Pharmacologic reversion of epigenetic silencing of the PRKD1 promoter blocks breast tumor cell invasion and metastasis., Breast Cancer Res. 2013 Aug 23;15(2):R66., 23971832 |
| Supplier | Everest Biotech |
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