Goat anti-PTEN Antibody

Category: Antibodies
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EB06544
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Product Name Goat anti-PTEN Antibody
Synonyms PTEN; BZS; MHAM; TEP1; MMAC1; PTEN1; MGC11227; phosphatase and tensin homolog (mutated in multiple advanced cancers 1); tensin homolog; mutated in multiple advanced cancers 1; MMAC1 phosphatase and tension homolog deleted on chromosome 10; phosphatase and
Host Goat
Clonality Polyclonal
Conjugate Unconjugated
Immunogen CNEPFDEDQHTQIT
Molecular Weight 47.1
Target PTEN
Amino Acid Sequence CNEPFDEDQHTQIT
Reactivity Bovine, Canine, Human, Mouse, Porcine, Rat
Applications ELISA, IP, WB
Working Dilutions Peptide ELISA: antibody detection limit dilution 1:32000.
Form Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Diluent Buffer Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Reconstitution WB: Approx 55kDa band observed in lysates of cell line A431 and 50kDa in lysates of cell line NIH3T3 (calculated MW of 47.1kDa according to Human NP_000305 and Mouse NP_032986.1). Recommended concentration: 1-3µg/ml.. Notes: Immunoprecipitation:This antibody was deemed fit for IP under native conditions (observations from a customer). Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2
Gene Id Human GeneIDs: 5728
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
References Cantley LC, Neel BG. , New insights into tumor suppression: PTEN suppresses tumor formation by restraining the phosphoinositide 3-kinase/AKT pathway., Proc Natl Acad Sci U S A. 1999 Apr 13;96(8):4240-5. Review. , PMID: 10200246
Supplier Everest Biotech

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