| Product Name | Goat anti-SDF4 (aa161-175) Antibody |
|---|---|
| Synonyms | SDF4; stromal cell derived factor 4; Cab45; 45 kDa calcium-binding protein; OTTHUMP00000001754; SDF-4; calcium binding protein; stromal cell-derived factor 4 |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | C-EYKVKFLASKGHSEK |
| Molecular Weight | 39.6; 41.8 |
| Target | SDF4 (aa161-175) |
| Amino Acid Sequence | EYKVKFLASKGHSEK |
| Reactivity | Bovine, Canine, Human, Mouse, Rat |
| Applications | ELISA, FC, IF, IHC, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:64000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Approx 45kDa band observed in lysates of cell lines Jurkat, HeLa and U251 (calculated MW of 41.8kDa according to NP_057260.2). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.. IHC: In paraffin embedded Human Kidney shows preferential staining in the brush borders of epithelial cells. Recommended concentration: 5-10µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the golgi and cytoplasm of U2OS cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: |
| Gene Id | Human GeneIDs: 51150. Non-Human GeneIDs: 20318 (mouse); 155173 (rat); |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Kang H, Escudero-Esparza A, Douglas-Jones A, Mansel RE, Jiang WG, , Transcript analyses of stromal cell derived factors (SDFs): SDF-2, SDF-4 and SDF-5 reveal a different pattern of expression and prognostic association in human breast cancer., International journal of oncology 2009 Jul 35 (1): 205-11., PMID: 19513569 |
| Supplier | Everest Biotech |
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