Product Name | Goat anti-Semaphorin 5A Antibody |
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Synonyms | SEMA5A; sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A; HGNC:10736; SEMAF; semF; sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane |
Host | Goat |
Clonality | Polyclonal |
Conjugate | Unconjugated |
Immunogen | DEATKKACYSKGKS |
Molecular Weight | 121 |
Target | Semaphorin 5A |
Amino Acid Sequence | DEATKKACYSKGKS |
Reactivity | Bovine, Canine, Human, Mouse, Rat |
Applications | ELISA, IHC |
Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:128000. |
Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Reconstitution | WB: Preliminary experiments gave an approx 90kDa in Mouse Brain lysates and approx 25kDa band in Human Brain (Cerebellum), Colon, Heart and Kidney lysates after 0.03µg/ml antibody staining. Please note that currently we cannot find an explanation in the liter. IHC: In paraffin embedded Human Kidney shows membanous and vesicular staining in PCT cells. Recommended concentration: 4-6µg/ml. |
Gene Id | Human GeneIDs: 9037. Non-Human GeneIDs: 20356 (mouse); 310207 (rat); |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
References | Maraganore DM, de Andrade M, Lesnick TG, Strain KJ, Farrer MJ, Rocca WA, Pant PV, Frazer KA, Cox DR, Ballinger DG., High-resolution whole-genome association study of Parkinson disease, Am J Hum Genet. 2005 Nov;77(5):685-93. Epub 2005 Sep 9., PMID: 16252231 |
Supplier | Everest Biotech |
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