| Product Name | Goat anti-Sprouty Antibody |
|---|---|
| Synonyms | SPRY1; hSPRY1; sprouty homolog 1, antagonist of FGF signaling (Drosophila); sprouty (Drosophila) homolog 1 (antagonist of FGF signaling); sprouty, Drosophila, homolog of, 1 (antagonist of FGF signaling) |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | CPSRGQGKPS |
| Molecular Weight | 35.1; 35.1 |
| Target | Sprouty |
| Amino Acid Sequence | CPSRGQGKPS |
| Reactivity | Human |
| Applications | ELISA, FC, IF, IHC, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:16000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Approx 37kDa band observed in Human Kidney lysates (calculated MW of 35.1kDa according to NP_005832.1 ). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature. This.product has been successfully used in ERMS cells (PMID: 20. IHC: This product has been successfully used by customers in paraffin embedded Mouse Brain, showing nuclear staining. Recommended concentration: 1-3µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm and Golgi apparatus of HeLa and HepG2 cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of HEK293 cells. Rec |
| Gene Id | Human GeneIDs: 10252 |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Hanafusa H, Torii S, Yasunaga T, Matsumoto K, Nishida E. , Shp2, an SH2-containing protein-tyrosine phosphatase, positively regulates receptor tyrosine kinase signaling by dephosphorylating and inactivating the inhibitor Sprouty., J Biol Chem. 2004 May 28;279(22):22992-5., PMID: 15031289 |
| Supplier | Everest Biotech |
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