| Product Name | Goat anti-SSA1 / Ro52 Antibody |
|---|---|
| Synonyms | TRIM21; tripartite motif-containing 21; RNF81, RO52, SSA, SSA1; 52kD Ro/SSA autoantigen; 52kDa ribonucleoprotein autoantigen SS-A/Ro; Sicca syndrome antigen A; Sjogren syndrome antigen A1 (52kD, ribonucleoprotein autoantigen SS-A/Ro); Sjogren syndrome ant |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | CPLNIGSQGSTDY |
| Molecular Weight | 54.2 |
| Target | SSA1 / Ro52 |
| Amino Acid Sequence | CPLNIGSQGSTDY |
| Reactivity | Human |
| Applications | ELISA, FC, IF, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:16000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: In transfected HEK293 transiently expressing full-length Human TRIM21 (myc and DYKDDDDK tagged), a band of approx. 60kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommend. Notes: Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U2OS cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration |
| Gene Id | Human GeneIDs: 6737 |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Chan EK, Di Donato F, Hamel JC, Tseng CE, Buyon JP. , 52-kD SS-A/Ro: genomic structure and identification of an alternatively spliced transcript encoding a novel leucine zipper-minus autoantigen expressed in fetal and adult heart., J Exp Med. 1995 Oct 1;182(4):983-92., PMID: 7561701 |
| Supplier | Everest Biotech |
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