| Product Name | Goat anti-STAT3 (isoform 1 and 2) Antibody |
|---|---|
| Synonyms | STAT3; signal transducer and activator of transcription 3 (acute-phase response factor); APRF; DNA-binding protein APRF; acute-phase response factor; FLJ20882; MGC16063; HIES |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | DMELTSECATSPM |
| Molecular Weight | 88.0; 88.1 |
| Target | STAT3 (isoform 1 and 2) |
| Amino Acid Sequence | DMELTSECATSPM |
| Reactivity | Bovine, Human, Mouse, Rat |
| Applications | ELISA, FC, IF, IHC |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:8000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Preliminary testing showed a band at approx 80-85kDa in A431 nuclear cell lysate after 2µg/ml antibody staining (calculated MW of 80kDa according to NP_003141.2; and NP_644805.1). Primary incubation 1 hour at room temperature.. IHC: In paraffin embedded Mouse Thymus shows preferential staining of medula over cortex. Recommended concentration: 4-6µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and A431 cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: |
| Gene Id | Human GeneIDs: 6774. Non-Human GeneIDs: 20848 (mouse); 25125 (rat); |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Bromberg JF, Wrzeszczynska MH, Devgan G, Zhao Y, Pestell RG, Albanese C, Darnell JE Jr., Stat3 as an oncogene., Cell. 1999 Aug 6;98(3):295-303. Erratum in: Cell 1999 Oct 15;99(2):239., PMID: 10458605 |
| Supplier | Everest Biotech |
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