| Product Name | Goat anti-TBK1 (aa514-527) Antibody |
|---|---|
| Synonyms | TBK1; TANK-binding kinase 1; FLJ11330; NAK; T2K; NF-kB-activating kinase; NF-kappa-B-activating kinase; serine/threonine-protein kinase TBK1 |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | C-TIETSLQDIDSRLS |
| Molecular Weight | 83.6 |
| Target | TBK1 (aa514-527) |
| Amino Acid Sequence | TIETSLQDIDSRLS |
| Reactivity | Human |
| Applications | ELISA, FC, IF, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:32000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Approx 80kDa band observed in lysates of cell line LNCaP and A431 (calculated MW of 83.6kDa according to NP_037386.1). Recommended concentration: 0.5-1µg/ml. Primary incubation 1 hour at room temperature.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and in the cytoplasm and ER of HeLa cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. |
| Gene Id | Human GeneIDs: 29110 |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Parvatiyar K, Barber GN, Harhaj EW., TAX1BP1 and A20 inhibit antiviral signaling by targeting TBK1-IKKi kinases., J Biol Chem. 2010 May 14;285(20):14999-5009., PMID: 20304918 |
| Supplier | Everest Biotech |
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