| Product Name | Goat anti-Thrombospondin Antibody |
|---|---|
| Synonyms | THBS1; thrombospondin 1; THBS; TSP; TSP1; thrombospondin-1p180 |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | NRIPESGGDNSVFD-C |
| Molecular Weight | 129 |
| Target | Thrombospondin |
| Amino Acid Sequence | NRIPESGGDNSVFD |
| Reactivity | Bovine, Human, Mouse, Rat |
| Applications | ELISA, FC, IF, IHC, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:128000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Approx 150kDa band observed in Mouse Kidney lysates (calculated MW of 129kDa according to NP_035710.2). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.. IHC: Paraffin embedded Human Spleen. Recommended concentration: 5µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and HepG2 cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration |
| Gene Id | Human GeneIDs: 7057 |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Pan MR, Chang HC, Chuang LY, Hung WC., The nonsteroidal anti-inflammatory drug NS398 reactivates SPARC expression via promoter demethylation to attenuate invasiveness of lung cancer cells., Exp. Biol. Med. (Maywood) 2008 Apr 233 (4): 456-62., PMID: 18367635 |
| Supplier | Everest Biotech |
All Research Products are sold for laboratory RESEARCH USE ONLY and ARE NOT TO BE USED FOR HUMAN OR ANIMAL THERAPEUTIC OR DIAGNOSTIC APPLICATIONS. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. ARP American Research Products, Inc. does not submit its products for regulatory review by any government body or other organization, and we do not validate them for clinical, therapeutic or diagnostic use, or for safety and effectiveness. You are solely responsible for making sure that the way you use the products complies with applicable laws, regulations and governmental policies and for obtaining all necessary approvals, intellectual property rights, licenses and permissions that you may need related to your use. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process. ARP American Research Products, Inc. disclaims any and all representations or warranties of any kind whatsoever, express or implied, including without limitation any implied warranties of merchantability or fitness for a particular purpose, of non-infringement, or regarding results obtained through the use of any product, whether arising from a statute or otherwise in law or from a course of performance, dealing or usage of trade.
