Goat anti-TRAF2 Antibody

Category: Antibodies
Catalog
EB07171
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Product Name Goat anti-TRAF2 Antibody
Synonyms TRAF2; TNF receptor-associated factor 2 ; MGC:45012; TRAP; TRAP3 ; OTTHUMP00000064745; tumor necrosis factor type 2 receptor associated protein 3
Host Goat
Clonality Polyclonal
Conjugate Unconjugated
Immunogen C-KMEAKNSYVRDD
Molecular Weight 55.9
Target TRAF2
Amino Acid Sequence KMEAKNSYVRDD
Reactivity Bovine, Canine, Human, Mouse, Rat
Applications ELISA, FC, IF, IHC, WB
Working Dilutions Peptide ELISA: antibody detection limit dilution 1:128000.
Form Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Diluent Buffer Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Reconstitution WB: Approx 50kDa band observed in Human Ovary and Mouse Testis lysates and in lysates of cell lines HepG2 and Jurkat. Preliminary testing also showed a band at approx 50kDa in U937, NIH3T3 and HEK293 cell lysates (calculated MW of 55.9kDa; according to Human . IHC: Paraffin embedded Human Pancreas, Kidney and Placenta. Recommended concentration: 3.75µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulums of HeLa and A431 cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended co
Gene Id Human GeneIDs: 7186. Non-Human GeneIDs: 22030 (mouse);
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
References Xu Y, Huang S, Liu ZG, Han J. , Poly(ADP-ribose) polymerase-1 signaling to mitochondria in necrotic cell death requires RIP1/TRAF2-mediated JNK1 activation., J Biol Chem. 2006 Jan 30; [Epub ahead of print] , PMID: 16446354
Supplier Everest Biotech

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