| Product Name | Goat anti-TRIM5 / RNF88 (alpha isoform) Antibody |
|---|---|
| Synonyms | TRIM5; tripartite motif-containing 5 ; HGNC:16276; RNF88; tripartite motif protein 5; tripartite motif protein TRIM5; TRIM5alpha; OTTHUMP00000069812; tripartite motif protein TRIM; tripartite motif-containing protein 5 alpha |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | CNIEKNENYQPK |
| Molecular Weight | 56.3 |
| Target | TRIM5 / RNF88 (alpha isoform) |
| Amino Acid Sequence | CNIEKNENYQPK |
| Reactivity | Human |
| Applications | ELISA, IHC |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:32000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Preliminary experiments gave an approx 37kDa band in Lysates of Human cell lines Daudi, Jurkat and MOLT4 after 0.3 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calcul. IHC: In paraffin embedded Human Kidney shows cytoplasm staining in PCT. Recommended concentration, 3-6µg/ml. |
| Gene Id | Human GeneIDs: 85363 |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Berthoux L, Sebastian S, Sokolskaja E, Luban J. , Cyclophilin A is required for TRIM5{alpha}-mediated resistance to HIV-1 in Old World monkey cells., Proc Natl Acad Sci U S A. 2005 Oct 11;102(41):14849-53. Epub 2005 Oct 3. , PMID: 16203999 |
| Supplier | Everest Biotech |
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