Goat anti-Triosephosphate isomerase Antibody

Category: Antibodies
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EB06643
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Product Name Goat anti-Triosephosphate isomerase Antibody
Synonyms TPI1; triosephosphate isomerase 1; TPI; MGC88108
Host Goat
Clonality Polyclonal
Conjugate Unconjugated
Immunogen C-LKPEFVDIINAKQ
Molecular Weight 26.7; 30.8; 18.0
Target Triosephosphate isomerase
Amino Acid Sequence LKPEFVDIINAKQ
Reactivity Bovine, Canine, Human, Mouse, Rat
Applications ELISA, FC, IHC, WB
Working Dilutions Peptide ELISA: antibody detection limit dilution 1:16000.
Form Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Diluent Buffer Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Reconstitution WB: Approx 26kDa band observed in lysates of cell lines HEK293, HeLa, HepG2, Jurkat, Raw264.7. KNRK and MDCK (calculated MW of 26.7kDa according to Human NP_000356.1). Recommended concentration: 0.001-0.01µg/ml. Primary incubation 1 hour at room temperature. . IHC: In paraffin embedded Human Liver shows staining of the sinusoids. Recommended concentration: 2-4µg/ml. Paraffin embedded Human Pancreas. Recommended concentration: 5µg/ml.. Notes: Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.
Gene Id Human GeneIDs: 7167. Non-Human GeneIDs: 21991 (mouse); 24849 (rat);
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
References Jiang PZ, Gan M, Huang H, Shen XM, Wang S, Yao KT. , Proteomics-based Identification of Proteins with Altered Expression Induced by 12-O-Tetradecanoylphorbol 13-acetate in Nasopharyngeal Carcinoma CNE2 Cells., Acta Biochim Biophys Sin (Shanghai). 2005 Feb;37(2):97-106. , PMID: 15685366
Supplier Everest Biotech

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