| Product Name | Goat anti-UBC3B Antibody |
|---|---|
| Synonyms | UBE2R2; UBC3B; CDC34B; FLJ20419; MGC10481; ubiquitin-conjugating enzyme E2R 2; ubiquitin-conjugating enzyme UBC3B |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | AQQQMTSSQKALM-C |
| Molecular Weight | 27 |
| Target | UBC3B |
| Amino Acid Sequence | AQQQMTSSQKALM |
| Reactivity | Human, Mouse, Rat |
| Applications | ELISA, IEM, IHC |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:4000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: No signal obtained yet but low background observed in Human Testis and U937 lysates at up to 1µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?. IHC: In paraffin embedded Human Testis shows textured nuclear staining of the primary spermatocytes . Recommended concentration, 2-4µg/ml.. Notes: Immunoelectron microscopy: This product stained chromatoid bodies in Rat spermatids. |
| Gene Id | Human GeneIDs: 54926. Non-Human GeneIDs: 67615 (mouse); |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Semplici F, Meggio F, Pinna LA, Oliviero S. , CK2-dependent phosphorylation of the E2 ubiquitin conjugating enzyme UBC3B induces its interaction with beta-TrCP and enhances beta-catenin degradation., Oncogene. 2002 Jun 6;21(25):3978-87., PMID: 12037680 |
| Supplier | Everest Biotech |
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