| Product Name | Goat anti-UBE2C / UBCH10 Antibody |
|---|---|
| Synonyms | UBE2C; UBCH10; dJ447F3.2; ubiquitin-conjugating enzyme E2C; ubiquitin-protein ligase C; ubiquitin carrier protein E2-C; cyclin-selective ubiquitin carrier protein; mitotic-specific ubiquitin-conjugating enzyme; RP3-447F3.1 |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | C-QETYSKQVTSQEP |
| Molecular Weight | 19.7; 17.4; 16.7; 15.8; 14.3 |
| Target | UBE2C / UBCH10 |
| Amino Acid Sequence | QETYSKQVTSQEP |
| Reactivity | Human |
| Applications | ELISA, FC, IF, IP, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:32000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Approx.19KDa band observed in lysates of cell lines HeLa and HEK293 (calculated MW of 19.7kDa according to NP_008950.1). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and MCF7 cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: |
| Gene Id | Human GeneIDs: 11065 |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Townsley FM, Aristarkhov A, Beck S, Hershko A, Ruderman JV., Dominant-negative cyclin-selective ubiquitin carrier protein E2-C/UbcH10 blocks cells in metaphase., Proc Natl Acad Sci U S A 1997 Mar 18;94(6):2362-7, PMID: 9122200 |
| Supplier | Everest Biotech |
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