| Product Name | Goat anti-Vimentin Antibody |
|---|---|
| Synonyms | VIM; vimentin; FLJ36605 |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | C-QVINETSQHHDDLE |
| Molecular Weight | 53.7 |
| Target | Vimentin |
| Amino Acid Sequence | QVINETSQHHDDLE |
| Reactivity | Bovine, Canine, Human, Mouse, Porcine, Rat |
| Applications | ELISA, FC, IF, IHC, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:4000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Approx 55kDa band observed in lysates of cell line HeLa and Jurkat and in Mouse Ovary lysates, and approx. 55-60kDa band in Rat Ovary lysates (calculated MW of 53.7kDa according to Human NP_003371.2, Mouse NP_035831.2 and Rat NP_112402.1). Recommended con. IHC: Paraffin embedded Human Kidney. Recommended concentration: 5µg/ml.. Notes: Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. Immunofluorescence: Strong expression of the protein seen in the cytoplasm/Intermediate filaments of U2OS cells. Recommended conc |
| Gene Id | Human GeneIDs: 7431. Non-Human GeneIDs: 22352 (mouse); 81818 (rat); |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Maraina CH, Nurdayana AK, Rusni D, Azwany Y., Diagnostic value of anti-modified citrullinated vimentin in rheumatoid arthritis., Int J Rheum Dis. 2010 Oct;13(4):335-9., PMID: 21199468 |
| Supplier | Everest Biotech |
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