| Product Name | Goat anti-VPS35 / MEM3 Antibody |
|---|---|
| Synonyms | VPS35; MEM3; FLJ10752; FLJ13588; FLJ20388; DKFZp434E1211; DKFZp434P1672; vacuolar protein sorting 35 (yeast); maternal-embryonic 3; vacuolar protein sorting 35 homolog (S. cerevisiae); vacuolar protein sorting 35 |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | C-SPESEGPIYEGLIL |
| Molecular Weight | 91.7 |
| Target | VPS35 / MEM3 |
| Amino Acid Sequence | SPESEGPIYEGLIL |
| Reactivity | Bovine, Human, Mouse, Rat |
| Applications | ELISA, IF, IHC, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:128000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Approx 90kDa band observed in Human (Cerebellum), Mouse and Rat Brain lysates and in lysates of cell line HepG2, and approx. 85kDa observed in lysates of cell line HEK293 (calculated MW of 91.7kDa according to Human NP_060676.2, Mouse NP_075373.1). Reco. IHC: Paraffin embedded Human Prostate. Recommended concentration: 8µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm/vesicles of HEK293 and U2OS cells. Recommended concentration: 10µg/ml. |
| Gene Id | Human GeneIDs: 55737. Non-Human GeneIDs: 65114 (mouse); |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Zhang P, Yu L, Gao J, Fu Q, Dai F, Zhao Y, Zheng L, Zhao S. , Cloning and characterization of human VPS35 and mouse Vps35 and mapping of VPS35 to human chromosome 16q13-q21., Genomics. 2000 Dec 1;70(2):253-7., PMID: 11112353 |
| Supplier | Everest Biotech |
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