| Product Name | Goat anti-WNT4 Antibody |
|---|---|
| Synonyms | WNT4; wingless-type MMTV integration site family, member 4; WNT-4; WNT-4 protein precursor; RP1-224A6.7; SERKAL; WNT-4 protein |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | C-SNWLYLAKLSSVGS |
| Molecular Weight | 39.1 |
| Target | WNT4 |
| Amino Acid Sequence | SNWLYLAKLSSVGS |
| Reactivity | Human, Mouse, Rat |
| Applications | ELISA, IF, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:4000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Approx 40+35kDa band observed in lysates of cell line Jurkat (calculated MW of 39.1kDa according to NP_110388.2). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature. Negative Control: U937 cell lysate.. Notes: Immunofluorescence: Strong expression of the protein seen in the vesicles of HeLa and MCF7 cells. Recommended concentration: 10µg/ml. |
| Gene Id | Human GeneIDs: 54361. Non-Human GeneIDs: 22417 (mouse); |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Huguet EL, McMahon JA, McMahon AP, Bicknell R, Harris AL., Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell., Cancer Res. 1994 May 15;54(10):2615-21., PMID: 8168088 |
| Supplier | Everest Biotech |
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