| Product Name | Goat anti-ZAP70 Antibody |
|---|---|
| Synonyms | ZAP70; zeta-chain (TCR) associated protein kinase 70kDa; FLJ17670; FLJ17679; SRK; STD; TZK; ZAP-70; 70 kDa zeta-associated protein; syk-related tyrosine kinase; tyrosine-protein kinase ZAP-70; zeta-chain associated protein kinase, 70kD |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | C-SDGYTPEPARITSPD |
| Molecular Weight | 69.9; 35.6 |
| Target | ZAP70 |
| Amino Acid Sequence | SDGYTPEPARITSPD |
| Reactivity | Bovine, Canine, Human, Mouse, Rat |
| Applications | ELISA, IP, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:32000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Approx 70kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 69.9kDa according to NP_001070.2). Recommended concentration: 0.3-1µg/ml.. Notes: Immunoprecipitation:This antibody was deemed fit for IP under native conditions (observations from customer). Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (201 |
| Gene Id | Human GeneIDs: 7535. Non-Human GeneIDs: 22637 (mouse); 301348 (rat); |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Crespo M, Bosch F, Villamor N, Bellosillo B, Colomer D, Rozman M, Marcé S, López-Guillermo A, Campo E, Montserrat E., ZAP-70 expression as a surrogate for immunoglobulin-variable-region mutations in chronic lymphocytic leukemia., N Engl J Med. 2003 May 1;348(18):1764-75., PMID: 12724482 |
| Supplier | Everest Biotech |
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