GPX1 ELISA kit (Rat)

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SEA295Ra
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Product Name GPX1 ELISA kit (Rat)
Description This assay has high sensitivity and excellent specificity for detection of Glutathione Peroxidase 1 (GPX1). No significant cross-reactivity or interference between Glutathione Peroxidase 1 (GPX1) and analogues was observed.
Synonyms GSHPX1; Cellular glutathione peroxidase
Method Sandwich
Detection Range 1.56-100ng/mL
Sensitivity 0.61ng/mL
Target GPX1
Specificity This assay has high sensitivity and excellent specificity for detection of Glutathione Peroxidase 1 (GPX1). No significant cross-reactivity or interference between Glutathione Peroxidase 1 (GPX1) and analogues was observed.
Reactivity Rat
Sample Types Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids
Reconstitution 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately.
Uniprot P04041
Storage 4°C for 1 month/ -20°C for long-term(within shelf life)
Notes Colormetric
References P2×7?Receptor in the Kidneys of Diabetic Rats Submitted to Aerobic Training or to N-Acetylcysteine Supplementation;Consequences of age on ischemic wound healing in rats: altered antioxidant activity and delayed wound closure;Evaluation of hepatorenal impairments in Wistar rats coexposed to low-dose lead, cadmium and manganese: insights into oxidative stress mechanism;Reduced Dietary Selenium Impairs Vascular Function by Increasing Oxidative Stress in Sprague-Dawley Rat Aortas.;Dyslipdemia induced by chronic low dose co-exposure to lead, cadmium and manganese in rats: the role of oxidative stress;Influence of deficient nutrition on trace element status and antioxidant defense system;Rapamycin improves renal injury induced by Iodixanol in diabetic rats by deactivating the mTOR/p70S6K signaling pathway;AMELIORATIVE EFFECT OF AQUEOUS EXTRACT OF HIBISCUS SABDARIFFA (ROSELLE) ON SALT-INDUCED HYPERTENSION IN WISTAR RATS;Effects of Ganoderma lucidum polysaccharides on different pathways involved in the development of spinal cord ischemia reperfusion injury: biochemical ¡­;Intake of Bifidobacterium lactis Probio-M8 fermented milk protects against alcoholic liver disease;A Famous Chinese Medicine Formula: Yinhuo Decoction Antagonizes the Damage of Corticosterone to PC12 Cells and Improves Depression by Regulating …;
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