| Product Name | GrpE, Recombinant |
|---|---|
| Description | GrpE ,co-chaperone of E.coli, participates actively in the response to hyperosomotic and heat shock by preventing the aggregation of stress-denatured proteins in association with DnaK. This protein is the nucleotide exchange factor for DnaK and may function as a thermosensor. Several rounds of ATP-dependent interactions between DnaJ, Dna K and GrpE are required for fully efficient folding. Recombinant GrpE protein was overexpressed in E.coli and purified by using the conventional column chromatography techniques. |
| Synonyms | HSP-70 cofactor, Heat shock protein B25.3, HSP24, GrpE, HSP 70 cofactor. |
| Host | E. coli |
| Molecular Weight | 34.9kDa (315aa), confirmed by MALDI-TOF. |
| Amino Acid Sequence | MSSKEQKTPE GQAPEEIIMD QHEEIEAVEP EASAEQVDPR DEKIANLEAQ LAEAQTRERD GILRVKAEME NLRRRTELDI EKAHKFALEK FINELLPVID SLDRALEVAD KANPDMSAMV EGIELTLKSM LDVVRKFGVE VIAETNVPLD PNVHQAIAMV ESDDVAPGNV LGIMQKGYTL NGRTIRAAMV TVAKAKA |
| Tag | His-tag |
| Reactivity | E. coli |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris pH 8.0, 20% glycerol |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Lipinska B, King J, Ang D, Georgopoulos C (1988). Nucleic Acids Res.16: 545-7562 Liberek K., Marszzalek J., Ang D, Georgopoulos C., Zylicz M. (1991). Proc. Natl. Acad.Sci. U.S.A. 88: 2874-2878. |
| Background | GrpE ,co-chaperone of E.coli, participates actively in the response to hyperosomotic and heat shock by preventing the aggregation of stress-denatured proteins in association with DnaK. This protein is the nucleotide exchange factor for DnaK and may function as a thermosensor. Several rounds of ATP-dependent interactions between DnaJ, Dna K and GrpE are required for fully efficient folding. Recombinant GrpE protein was overexpressed in E.coli and purified by using the conventional column chromatography techniques. |
| Supplier | ARP |
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