| Product Name | GTF3C6, 1-213aa, Human |
|---|---|
| Description | RNA polymerases are unable to initiate RNA synthesis in the absence of additional proteins called general transcription factors (GTFs). GTFs assemble in a complex on the DNA promoter and recruit the RNA polymerase. GTF3C family proteins (e.g., GTF3C1, MIM 603246) are essential for RNA polymerase III to make a number of small nuclear and cytoplasmic RNAs, including 5S RNA (MIM 180420), tRNA, and adenovirus-associated (VA) RNA of both cellular and viral origin. Recombinant human GTF3C6 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | General transcription factor 3C polypeptide 6, General transcription factor 3C polypeptide 6, bA397G5.3, C6orf51, TFIIIC35 |
| Host | E. coli |
| Molecular Weight |
9.3 kDa (93aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher) |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSMAAAADE RSPEDGEDEE EEEQLVLVEL SGIIDSDFLS KCENKCKVLG IDTERPILQV DSCVFAGEYE DTLGTCVIFE ENVEHADTEG NNKTVLKYKC HTMKKLSMTR TLLTEKKEGE ENIGGVEWLQ IKDNDFSYRP NMICNFLHEN EDEEVVASAP DKSLELEEEE IQMNDSSNLS CEQEKPMHLE IEDSGPLIDI PSETEGSVFM ETQMLP |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in Phosphate buffer saline (pH 7.4) containing 10% glycerol |
| Concentration | 0.25 mg/ml (determined by BCA assay) |
| Purity | > 85% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Dumay-Odelot,H., et al. (2007) J. Biol. Chem. 282 (23), 17179-17189 |
| Background | RNA polymerases are unable to initiate RNA synthesis in the absence of additional proteins called general transcription factors (GTFs). GTFs assemble in a complex on the DNA promoter and recruit the RNA polymerase. GTF3C family proteins (e.g., GTF3C1, MIM 603246) are essential for RNA polymerase III to make a number of small nuclear and cytoplasmic RNAs, including 5S RNA (MIM 180420), tRNA, and adenovirus-associated (VA) RNA of both cellular and viral origin. Recombinant human GTF3C6 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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