| Product Name | HA ELISA kit (General species) |
|---|---|
| Description | This assay has high sensitivity and excellent specificity for detection of Histamine (HA). No significant cross-reactivity or interference between Histamine (HA) and analogues was observed. |
| Synonyms | Histamin |
| Method | Competitive |
| Detection Range | 390.6-100,000pg/mL |
| Sensitivity | 0.52ng/mL |
| Target | HA |
| Specificity | This assay has high sensitivity and excellent specificity for detection of Histamine (HA). No significant cross-reactivity or interference between Histamine (HA) and analogues was observed. |
| Sample Types | Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids |
| Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix.Incubate 1 hour at 37℃; 3.Aspirate and wash 3 times; 4.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 5.Aspirate and wash 5 times; 6.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 7.Add 50uL Stop Solution.Read at 450 nm immediately. |
| Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
| Notes | Colormetric |
| References | PEST-domain-enriched tyrosine phosphatase and glucocorticoids as regulators of anaphylaxis in mice;Comparison of sensitization between β-lactoglobulin and its hydrolysates;Integrative analysis of proteomics and metabolomics of anaphylactoid reaction induced by Xuesaitong injection;Expression of inducible nitric oxide synthase in mast cells contributes to the reCavia (Guinea pig )lation of inflammatory cytokines in irritable bowel syndrome with diarrhea;Induction of immune responses and allergic reactions in piglets by injecting glycinin;Effects of Dectin-1 on the mast cells in allergic conjunctivitis and its underlying mechanism;Proteomics Study on Nonallergic Hypersensitivity Induced by Compound 4880 and Ovalbumin;Evaluation of anaphylactoid constituents in vitro and in vivo.;Ultraviolet A photosensitivity profile of dexchlorpheniramine maleate and promethazine-based creams: Anti-inflammatory,antihistaminic, and skin barrier protection properties;Adipose Tissue-Derived Mesenchymal Stem Cell Modulates the Immune Response of Allergic Rhinitis in a Rat Model;Metabolomics analysis of baicalin on ovalbumin-sensitized allergic rhinitis rats;MOTION SICKNESS INDUCES PHYSIOLOGICAL AND NEURONAL ALTERATIONS IN MOUSE MODEL;Artemisia annua sublingual immunotherapy for seasonal allergic rhinitis: a randomized controlled trial;Animal models for analysis of hypersensitivity reactions to Shuanghuanglian injection;Protective effect of Asarum sieboldii essential oil on ovalbumin induced allergic rhinitis in rat;A vaccine-based nanosystem for initiating innate immunity and improving tumor immunotherapy;CCR3‑shRNA promotes apoptosis and inhibits chemotaxis and degranulation of mouse mast cells;Study of some hematological and immunological parameters associated with the infection of intestinal parasites in the holy city of Kerbala, Iraq;Pharmacokinetics and safety of TCMCB07, a melanocortin-4 antagonist peptide in dogs; |
| Supplier | Cloud Clone |
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