HBZ, 1-142aa, Human, Recombinant

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01-1813
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Proteins, Recombinant
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Product Name HBZ, 1-142aa, Human, Recombinant
Amino Acid Sequence MSLTKTERTI IVSMWAKIST QADTIGTETL ERLFLSHPQT KTYFPHFDLH PGSAQLRAHG SKVVAAVGDA VKSIDDIGGA LSKLSELHAY ILRVDPVNFK LLSHCLLVTL AARFPADFTA EAHAAWDKFL SVVSSVLTEK YRLEHHHHHH
Applications SDS-PAGE
Background Hemoglobin subunit zeta (HBZ) is belongs to the globin family. Zeta-globin is alpha-like hemoglobin. The HBZ polypeptide is synthesized in the yolk sac of the early embryo, while alpha-globin is produced throughout fetal and adult life. The HBZ gene includes five functional genes and two pseudogenes. The order of genes is: 5' - zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 -alpha-1 - theta1 - 3'. Recombinant human HBZ fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Concentration 0.25 mg/ml (determined by Bradford assay)
Form Liquid, in 20 mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 0.15M NaCl
Species Human
Host E.coli
Intended Use For Research Use Only
Molecular Weight 78.4 kDa (677aa), confirmed by MALDI-TOF.
NCBI Accession # NP_005323
Purity > 90% by SDS-PAGE
References Lau ET., et al. (2001) Prenat. Diagn. 21(7): 529-39.
Kidd RD., et al. (2001) Biochemistry. 40(51):15669-75.
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Synonyms Zeta-globin, HBAZ, HBZ2, Hemoglobin subunit zeta HBZ 2, Hemoglobin zeta, Hemoglobin zeta chain, Zeta globin.
Tag His-tag

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