| Product Name | Hepc ELISA kit (Rat) |
|---|---|
| Description | This assay has high sensitivity and excellent specificity for detection of Hepcidin (Hepc). No significant cross-reactivity or interference between Hepcidin (Hepc) and analogues was observed. |
| Synonyms | HAMP; HFE2B; PLTR; LEAP1; Hepcidin Antimicrobial Peptide; Liver-expressed antimicrobial peptide 1; Putative liver tumor regressor |
| Method | Competitive |
| Detection Range | 1.23-100ng/mL |
| Sensitivity | 0.45ng/mL |
| Target | Hepc |
| Specificity | This assay has high sensitivity and excellent specificity for detection of Hepcidin (Hepc). No significant cross-reactivity or interference between Hepcidin (Hepc) and analogues was observed. |
| Reactivity | Rat |
| Sample Types | Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids |
| Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix.Incubate 1 hour at 37℃; 3.Aspirate and wash 3 times; 4.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 5.Aspirate and wash 5 times; 6.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 7.Add 50uL Stop Solution.Read at 450 nm immediately. |
| Uniprot | Q99MH3 |
| Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
| Notes | Colormetric |
| References | Hepcidin expression in colon during trinitrobenzene sulfonic acid-induced colitis in rats;Modulations of serum hepcidin, IL6 and iron status by different exercise regimens in ovarectomized rats;Inappropriate expression of hepcidin by liver congestion contributes to anemia and relative iron deficiency;The action of JAK, SMAD and ERK signal pathways on hepcidin suppression by polysaccharides from Angelica sinensis in rats with iron deficiency anemia;Changes in skeletal muscle iron metabolism outpace amyotrophic lateral sclerosis onset in transgenic rats bearing the G93A hmSOD1 gene mutation;Ferrous sulfate, but not iron polymaltose complex, aggravates local and systemic inflammation and oxidative stress in dextran sodium sulfate-induced colitis in rats;Kupffer cell depletion protects against the steatosis, but not the liver damage, induced by marginal copper, high fructose diet in male rats.;Silkworm feces extract improves iron deficiency anemia via suppressing hepcidin expression and promoting iron-regulatory proteins expression;Acute and Chronic Iron Overloading Differentially Modulates the Expression of Cellular Iron-homeostatic Molecules in Normal Rat Kidney;Dietary gelatin enhances non-heme iron absorption possibly via regulation of systemic iron homeostasis in rats; |
| Supplier | Cloud Clone |
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