| Product Name | HIF1a, 1-85aa, Human |
|---|---|
| Description | Hypoxia-inducible factor-1 (HIF-1), identified as one of the transcription factors, has been found to play an essential role in oxygen homeostasis. HIF-1 is a heterodimer composed of HIF-1 beta subunit and one of three subunits (Hif-1 alpha, Hif-2 alpha or Hif-3 alpha). The activation of Hif-1 alpha is closely associated with a variety of tumors and oncogenic pathways. Hif-1 alpha consists of DNA binding domain(DBD domain), dimerization domain and C-terminal regulatory domains, including two transactivation domains(TAD), an oxygen-dependent degradation(ODD) domain, and inhibitory domains. Recombinant human Hif-1 alpha (1-85) protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | BHLHE78, MOP1, PASD8, Hypoxia-inducible factor 1-alpha |
| Host | E.coli |
| Molecular Weight | 21.6 kDa (204aa), confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MEGAGGANDK KKISSERRKE KSRDAARSRR SKESEVFYEL AHQLPLPHNV SSHLDKASVM RLTISYLRVR KLLDAGDLDI EDDMK |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl. |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 80 % by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| References | Okuyama H., et al. (2006) J Biol Chem. 281(22):15554-63. |
| Supplier | ARP |
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