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|Product Name||HLA-DOA, 26-217aa, Human|
|Amino Acid Sequence||MGSSHHHHHH SSGLVPRGSH MGSTKADHMG SYGPAFYQSY GASGQFTHEF DEEQLFSVDL KKSEAVWRLP EFGDFARFDP QGGLAGIAAI KAHLDILVER SNRSRAINVP PRVTVLPKSR VELGQPNILI CIVDNIFPPV INITWLRNGQ TVTEGVAQTS FYSQPDHLFR KFHYLPFVPS AEDVYDCQVE HWGLDAPLLR HWELQVPIPP PDAME|
|Background||HLA-DOA belongs to the HLA class II alpha chain paralogues. HLA-DOA forms a heterodimer with HLA-DOB. The heterodimer, HLA-DO, is found in lysosomes in B cells and regulates HLA-DM-mediated peptide loading on MHC class II molecules. In comparison with classical HLA class II molecules, this gene exhibits very little sequence variation, especially at the protein level. Recombinant human HLA-DAO protein, fused to His-tag at N-terminus, was expressed in E.coli.|
|Concentration||0.5 mg/ml (determined by Bardford assay)|
|Form||Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT|
|Intended Use||For Research Use Only|
|Molecular Weight||41.4 kDa (357aa), confirmed by MALDI-TOF|
|NCBI Accession #||NP_002110|
|Purity||> 90% by SDS-PAGE|
|References||Yoon,T., et al. (2012) Proc. Natl. Acad. Sci. U.S.A. 109 (28), 11276-11281
Gieger,C., et al. (2012) Nature 480 (7376), 201-208
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Synonyms||HLA class II histocompatibility antigen, DO alpha chain, HLA-DNA, HLA-DZA, HLADZ|
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