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|Product Name||HLA-DRB1, 30-227aa Human|
|Amino Acid Sequence||MGSSHHHHHH SSGLVPRGSH MGDTRPRFLW QPKRECHFFN GTERVRFLDR YFYNQEESVR FDSDVGEFRA VTELGRPDAE YWNSQKDILE QARAAVDTYC RHNYGVVESF TVQRRVQPKV TVYPSKTQPL QHHNLLVCSV SGFYPGSIEV RWFLNGQEEK AGMVSTGLIQ NGDWTFQTLV MLETVPRSGE VYTCQVEHPS VTSPLTVEWR ARSESAQSK|
|Background||HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa. It is encoded by 6 exons. Exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail. Recombinant human HLA-DRB1 protein, fused to His-tag at N-terminus, was expressed in E.coli.|
|Concentration||0.5mg/ml (determined by Bradford assay)|
|Form||Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.4M Urea|
|Intended Use||For Research Use Only|
|Molecular Weight||50.5 kDa(455aa)|
|NCBI Accession #||NP_002115|
|Purity||> 85% by SDS-PAGE|
|References||Menendez-Benito V., et al. (2007) Immunity. 26:1-3
De Gassart A., et al. (2008) Proc. Natl. Acad. Sci. U.S.A. 105:3491-3496
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Synonyms||Major histocompatibility complex, class II, DR beta 1, DRB1, HLA DRB1, HLA-DR1B, HLA-DRB1|
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