| Product Name | HO1 ELISA kit (Rat) |
|---|---|
| Description | This assay has high sensitivity and excellent specificity for detection of Heme Oxygenase 1, Decycling (HO1). No significant cross-reactivity or interference between Heme Oxygenase 1, Decycling (HO1) and analogues was observed. |
| Synonyms | HMOX1; Hsp32; HMOX1D |
| Method | Sandwich |
| Detection Range | 0.156-10ng/mL |
| Sensitivity | 0.062ng/mL |
| Target | HO1 |
| Specificity | This assay has high sensitivity and excellent specificity for detection of Heme Oxygenase 1 (HO1). No significant cross-reactivity or interference between Heme Oxygenase 1 (HO1) and analogues was observed. |
| Reactivity | Rat |
| Sample Types | Serum, Plasma, Tissue Homogenatesand Other Biological Fluids |
| Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately. |
| Uniprot | P06762 |
| Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
| Notes | Colormetric |
| References | Sulforaphane protects against sodium valproate–induced acute liver injury;Carnosine ameliorates cognitive deficits in streptozotocin-induced diabetic rats: Possible involved mechanisms.;S-allyl cysteine ameliorates cognitive deficits in streptozotocin-diabetic rats via suppression of oxidative stress, inflammation, and acetylcholinesterase.;Ellagic acid exerts protective effect in intrastriatal 6-hydroxydopamine rat model of Parkinson's disease: Possible involvement of ERβ/Nrf2/HO-1 signaling.;Citrus leaf extract reduces blood pressure and vascular damage in repeatedly heated palm oil diet-Induced hypertensive rats.;Implication of Nrf2/HO-1 pathway in the coloprotective effect of coenzyme Q10 against experimentally induced ulcerative colitis.;Ameliorative potential of sitagliptin and/or resveratrol on experimentallyinduced clear cell renal cell carcinoma;Quercetin and tin protoporphyrin attenuate hepatic ischemia reperfusion injury: role of HO-1;Berberine ameliorates intrahippocampal kainate-induced status epilepticus and consequent epileptogenic process in the rat: Underlying mechanisms;Anti-hemolytic and anti-inflammatory activities of the methanolic extract of (P.Beauv.) Briq. leaves in 2-butoxyethanol-hemolytic induced …;Tempol relieves lung injury in a rat model of chronic intermittent hypoxia via suppression of inflammation and oxidative stress;Targeting Nrf2/HO-1 signaling by crocin: Role in attenuation of AA-induced ulcerative colitis in rats;Cilostazol against 2, 4, 6‐trinitrobenzene sulfonic acid‐induced colitis: Effect on tight junction, inflammation, and apoptosis;Crocin attenuates lung inflammation and pulmonary vascular dysfunction in a rat model of bleomycin-induced pulmonary fibrosis;The protective effect of Lactobacillus versus 5-aminosalicylic acid in ulcerative colitis model by modulation of gut microbiota and Nrf2/Ho-1 pathway;Isoliquiritigenin attenuates inflammation and modulates Nrf2/caspase-3 signalling in STZ-induced aortic injury; |
| Supplier | Cloud Clone |
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