| Product Name | HP, 19-347aa Human |
|---|---|
| Description | HP, as known as haptoglobin, is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. In blood plasma, haptoglobin binds free hemoglobin (Hb) released from erythrocytes with high affinity and thereby inhibits its oxidative activity. The haptoglobin-hemoglobin complex will then be removed by the reticuloendothelial system (mostly the spleen). Recombinant human HP protein, fused to His-tag at N-terminus, was expressed in E.coli |
| Synonyms | Haptoglobin isoform 2 preproprotein, BP, HP2ALPHA2, HPA1S |
| Host | E. coli |
| Molecular Weight | 25.1kDa (214aa) |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSVDSGNDV TDIADDGCPK PPEIAHGYVE HSVRYQCKNY YKLRTEGDGV YTLNNEKQWI NKAVGDKLPE CEAVCGKPKN PANPVQRILG GHLDAKGSFP WQAKMVSHHN LTTGATLINE QWLLTTAKNL FLNHSENATA KDIAPTLTLY VGKKQLVEIE KVVLHPNYSQ VDIGLIKLKQ KVSVNERVMP ICLPSKDYAE VGRVGYVSGW GRNANFKFTD HLKYVMLPVA DQDQCIRHYE GSTVPEKKTP KSPVGVQPIL NEHTFCAGMS KYQEDTCYGD AGSAFAVHDL EEDTWYATGI LSFDKSCAVA EYGVYVKVTS IQDWVQKTIA EN |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 0.4M UREA, 10% glycerol |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 80% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Dobryszycka W. et al. (1997) Eur J Clin Chem Clin Biochem. 35:647–654. Wassell J. et al. (2000) Clin. Lab. 46:547–552. |
| Background | HP, as known as haptoglobin, is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. In blood plasma, haptoglobin binds free hemoglobin (Hb) released from erythrocytes with high affinity and thereby inhibits its oxidative activity. The haptoglobin-hemoglobin complex will then be removed by the reticuloendothelial system (mostly the spleen). Recombinant human HP protein, fused to His-tag at N-terminus, was expressed in E.coli |
| Supplier | ARP |
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